endobj Chabi, N. W., C. T. R. Konfo, M. Adjagba, L. Moussedikou, E. Ahoussi-Dahouenon, A. Laleye, C. Gbaguidi, and M. M. Soumanou. 0000002992 00000 n The presence of these different compound groups depend on the polarity of the solvent used. Faraway (2002) reported that in the Biological Sciences when the coefficient of determination R2 ≥ 0.6, then the favorable results are attributable to the products used. 0000107047 00000 n In 7 d of exposure, methanol extract acted more rapidly (slope = 1.76 ± 0.14). 2011, Thakur 2012). The same tendency was also reported by Ojianwuna et al. About 870 million people in the world are undernourished because of inadequate intake of proteins, vitamins, and minerals in their diets (FAO 2012). While within 3 d of exposure, the hexane extract acted more rapidly (slope = 1.19 ± 0.13) than acetone and methanol extract. 2006, Demissie et al. 2000). At the lowest dosage (2 g/kg), acetone extract recorded less live insects (13.25) compared with hexane and methanol extracts with 19.00 and 52.00 live insects, respectively. Abbott’s formula (Abbot 1925) was used to correct control mortality when mortality in the control is comprised between 3 and 10% before submission to the ANOVA. Germination rate of cowpea seeds in treatments ranged from 28.75% in the untreated control to 47.50% with hexane extract, 13.75% in the untreated control to 60% with acetone extract, and 15.00% in the untreated control to 41.25% with methanol extract. 1. Also, the different concentration levels significantly protected the seeds with regard to seed damage caused by C. maculatus compared with the untreated control after 3 mo storage. Tagne Gabriel Fotso, Haman Katamssadan Tofel, Jean Pierre Abdou, Narcis Tchao, Calvain Monglo Zourmba, Cornel Adler, Elias Nchiwan Nukenine, Control of Callosobruchus maculatus (Coleoptera: Chrysomelidae) Using Fractionated Extracts from Cameroonian Hemizygia welwitschii (Lamiaceae) Leaf on Stored Vigna unguiculata (Fabales: Fabaceae), Journal of Insect Science, Volume 19, Issue 2, March 2019, 22, https://doi.org/10.1093/jisesa/iez029. Callosobruchus maculatus Fab. 0000009901 00000 n ��v̲@b�c�b��l���p�qP�4� _��I���F@�FS��1X0s3�3�`��t������"�5S�g� Among them, Hemizygia welwitschii, an herbaceous and annual plant, is widely present in tropical environment such as West, East, Central, and parts of southern Africa (Otieno et al. Unfortunately, the grain is extensively infested by a number of insect pests including Callosobruchus maculatus, which is a primary field to store pest causing considerably great losses to farmers (Ojo et al. Untreated controls for each set of treatments consisted of grains treated with 2 ml of pure respective solvents. 24 Females lay eggs on seed coat and larvae develop exclusively inside the seeds, at the expense of grain endosperm and embryo, and are responsible for cowpea damage. <>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Executive summary report, Interception of insect pests in exotic seed germplasm, Spinosad: a new natural product for stored grain protection, Bioactivity of novel botanical insecticide from, Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: possible mode of action against insect pests, Etude de l’extraction des composés phénoliques de différentes parties de la fleur d’artichaut (, A multivariate analysis of morphological variation in the, Insecticidal activity of the root extract of, Manuel de manipulation des semences dans les banques de gènes. Thank you for submitting a comment on this article. Mahama et al. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. 4 0 obj The moisture content (mc) of the seeds was determined according to the method used by AFNOR (1982). At the lower concentration (2 g/kg), the classification of extracts in terms of progeny inhibition was as follows: methanol extract > hexane extract > acetone extract with, respectively, 19.50, 19.75, and 36.75 C. maculatus emerged. 2006). This result is similar with the work of Umar (2008) who reported that the cowpea seeds remained viable which were treated with Jatropha curcas powder. In pest control, Fotso et al. (2015). The viability of seeds was not affected by the extracts. At this same time point, mortality recorded in cowpea seeds treated with acetone extract was lower and had no significant effect on adult mortality compared with untreated control. Complete percentage in terms of inhibition rates of C. maculatus adult was obtained by hexane extract from the dosage of 6 g/kg, while with methanol extract the percentages of reduction were 95.68 and 98.40% at 6 and 10 g/kg, respectively. 0000005710 00000 n The dried leaves were hand crushed into powder using locally made pestle and mortar. Des bio-essais étaient menés afin d’évaluer individuellement les activités insecticides des extraits à l’hexane, à l’acétone et au méthanol de la poudre des feuilles de Hemizygia welwitschii, contre Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae). Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon. (2018) and those of Danga et al. The extracts were applied at 2, 4, 6, and 10 g/kg of cowpea and the untreated seeds served as negative control. 0000081294 00000 n The grains were placed in moistened sand in perforated plastic plates. 0000009121 00000 n 0000004895 00000 n 0000002087 00000 n (2018) recently shown the efficacy of H. welwitschii leaves powder against C. maculatus and Sitophilus zeamais in stored cowpea and maize, respectively. has a wide range of use. Search for other works by this author on: Department of Biological Sciences, University of Bamenda, Bamenda, Cameroon, Center for Research on Medicinal Plants and Traditional Medicine (CRPMT), Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon, Julius Kühn-Institut, Institute for Ecological Chemistry, Plant Analysis and Stored Products Protection, Königin-Luise Str.19, Berlin, Germany, A method of computing the effectiveness of an insecticide, Preliminary phytochemical analysis and insecticidal activity of ethanolic extracts of four tropical plants (, Association Française de Normalisation (AFNOR), Recueil des normes françaises des produits dérivés des fruits et légumes. The solvent was removed after 48 h by filtration. This shows that H. welwitschii leaves powder extracts are highly effective in controlling bruchids infestations, which could be due to the presence of some bioactive compounds, including phenolic compounds, alkaloids, saponins, tannins, flavonoids, triterpenoids, and sterols. b�4aK��LW�90�g���"��I�+�Aed��,���A�4 0000002420 00000 n All the χ2 values were not significant for the three extracts at all the exposure periods, except for the hexane extract which was significant at 1 and 7 d of exposure. Please check for further notifications by email. It causes substantial quantitative and qualitative losses manifested by seed perforation and reductions in … Reduction in the F1 progeny emergence of C. maculatus in the Bambara groundnut treated with Ocimum canum Sims leaf extract fractions was also obtained by Kosini et al. In fact, the solvent polarity used and the solubility determined the nature of the compounds present in each plant extract (Mahmoudi et al. The finding obtained in this study revealed that, all the three solvent extracts were toxic against C. maculatus adults in the protection of cowpea (Vigna unguiculata) and having significant inhibition of F1 progeny emergence in treated grain. Association Française de Normalisation (AFNOR). Rao, N. K., J. Hanson, M. E. Dulloo, K. Ghosh, D. Nowell, and M. Larinde. 1 0 obj The subsequent progenies were used for all experimentations.