This suggests that they may play key roles in the responses to K88 in infected nematodes. 0000074604 00000 n 326 141 Vet Res. On the 2nd day of infection, the abundance of estB and elt in PR was 2.17 and 0.79 times lower than that in AT ( and ), respectively. 0000070438 00000 n The upper layer of bacterial liquid was transferred to new RNase-free tubes for the subsequent RNA extraction steps. The colonization ability of bacteria is related to many factors including pili, intestinal microenvironment, and colonization sites on intestinal cells [60–62]. Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs. 0000074683 00000 n Previous studies have shown that protein kinase A (PKA, kin-1) regulates various physiological activities involved in substrates through phosphorylation [83, 84]. Legend represents the log2 (fold change) of gene abundance; the redder the color, the higher the gene expression, and conversely, the bluer the color. 0000078189 00000 n 2 μL of each cDNA at 100 ng was included in 10 μL 2× iTaq Universal SYBR Green Supermix, 0.8 μL of each primer at 100 nM, and 6.4 μL ddH2O. Pediococcus were present in both healthy and infected cows. 0000073486 00000 n 0000170790 00000 n Laboratoire de Bactériologie-Virologie, Centre Hospitalier et Universitaire Pontchaillou, 35033, Rennes, France, J. M. Sire, P. Y. Donnio, R. Mesnard, P. Pouëdras & J. L. Avril, You can also search for this author in 0000171304 00000 n *�5�8Ў)���|�[Sd�0A8� 0000167164 00000 n HHS This study is aimed at exploring the mechanisms imparted by Pediococcus acidilactici P25 against ETEC K88 in … A 32-year-old male with a history of short gut syndrome following a small bowel transplant 1 year prior with severe rejection requiring explantation 3 weeks after transplant presented with fever (102°F), rigors, and dyspnea of 3 days' duration. Some genes’ abundances related to the innate immune response in C. elegans were significantly reduced due to K88 infection, but this was reversed by P25. Challenges of a one-health approach to the development of alternatives to antibiotics. 0000072129 00000 n Down and up represent the gene expression trend in PR vs. AT and AT vs. CK. Clec-39 and Clec-49 protein-deficient nematodes became sensitive to Serratia marcescens infection [69]. Several isolates of the Pediococcus acidilactici were found to produce the 466 0 obj <>stream 0000086943 00000 n The darker the color is, the greater the correlation is. Thank you for sharing this Journal of Clinical Microbiology article. Learn more about Institutional subscriptions. The pulmonary nodules disappeared at 1 month on the follow-up CT scan of the chest. Fan, “The role and regulation of catalase in respiratory tract opportunistic bacterial pathogens,”, H. S. Tehrani and A. 0000168664 00000 n T.; methodology, D.D. The contributions of the authors involved in this study are as follows: conceptualization, D.D. Hierarchical clustering analysis was performed based on the gene expression represented by the normalized RPKM value; the relationship between samples and genes were hierarchically clustered; and using the R software package (version 3.6.1), a heat map to present clustering results was generated. 0000076105 00000 n Analyses of acid production and acid and bile salt tolerance experiments of P25 used one-way ANOVA and Least Significant Difference (LSD) method for multiple comparisons in the SPSS software (version 24.0). In contrast, P25 had a certain anti-ETEC K88 colonization effect. J Anim Sci. Microbiol. Pediococcus acidilactici and Pediococcus pentosaceus are the most common species associated with opportunistic infections affecting compromised hosts. 0000074842 00000 n On the 4th day, the number of K88 in AT and PR was not significantly different (). 0000070594 00000 n Subscription will auto renew annually. Two reviews are available in the literature that report on rare cases of bacteremia in humans caused by Pediococcus … Get the latest public health information from CDC: P25 could colonize in the intestinal canal of nematodes. Clipboard, Search History, and several other advanced features are temporarily unavailable. In: Sneath PHA, Mair SN, Sharpe ME, Holt JG (ed): Bergey's manual of systematic bacteriology. 0000073303 00000 n In the blood, CD8(+) T cells were not affected by treatments or weaning. 0000077713 00000 n van der Meulen J, Hulst MM, Smits MA, Schuurman T. Benef Microbes. 0000154951 00000 n T.; drafting and revision of the manuscript, D.D. and K-Q. The extracted RNA was mixed with 2 μL 5× gDNA Eraser Buffer and 1 μL gDNA Eraser, and RNase-free dH2O was added to the total reaction volume of 10 μL. Therefore, our results could also further supplement that the inhibition of pathogens by LAB may be species diverse in host cell colonization. NLM A worm was considered dead when it repeatedly failed to respond to touch. In the present study, when P25 was mixed cultured with K88, not only the growth of K88 was significantly inhibited () but also the abundance of the enterotoxin genes estB and elt (Figure 4(d)). To monitor C. elegans lifespan, the number of living nematodes was recorded every day (the transfer of nematodes to the K88 suspension was considered on day 0) until all nematodes had died. So, the upregulation of the MAPK signaling pathway by P25 may also be essential to its protective effect against K88 in C. elegans. On days 4 and 6, there were no differences observed in either group (), but the abundances of estB and elt in PR were always lower than those in AT. European Journal of Clinical Microbiology and Infectious Diseases Mastro TD, Spika JS, Lozano P, Appel J, Facklam RR: Vancomycin-resistantPediococcus acidilactici: nine cases of bacteremia. (a) The statistical maps of differentially expressed genes (DEGs) upregulated and downregulated the situation among AT vs. CK and PR vs. AT. The results indicated that the differential expression patterns of genes relating to the MAPK pathway as measured by RNA-seq analysis followed the same trend in abundances detected by qRT-PCR (Figure 11), which showed that the RNA-seq results were reliable. Very few cases of documented infections due to this bacterium have been reported in the literature. 0000080641 00000 n 0000115086 00000 n The expression patterns of enterotoxin genes in the intestinal tract of C. elegans are shown in Figure 5. P. acidilactici is a homofermentative bacterium that can grow in a wide range of pH, temperature, and … The authors declare that there are no conflicts of interest regarding the publication of this paper. The method of extracting K88 RNA from nematodes in vivo was the same as previously described but with some modifications. 2007 May;66(2):260-8. doi: 10.1017/S0029665107005484. About Pediococcus acidilactici Pediococcus acidilactici is a facultative anaerobe bacteria. 0000075237 00000 n Charles C. Thomas, Springfield, IL, 1974, p. 81–97. The suspension was gradient diluted and plated onto EMB or MRS medium to determine the bacteria of the worm’s gut (). In the MLN, the percentage of CD8(+) T cells was not affected by any of the treatments at d 18 and 24 but decreased (P = 0.006) after weaning. 0000122390 00000 n The previous method was used to clean the bacteria from the surface of nematodes and break up the worms. The aqueous phase was collected, gently mixed with 600 μL isopropanol, and allowed to stand at 4°C for 30 min, then centrifuged at 12,000 rpm at 4°C for 15 min. Journal of Clinical Microbiology 1989, 27: 724–730. However, the proliferation of K88 was faster in AT and the abundance of K88 in worms of PR was significantly lower than that of AT (). Catalase (CAT, ctl-1 and ctl-2) has the function of regulating host defense to pathogens, apoptosis, aging, inflammation, tumor formation, and mutagenesis. We do not retain these email addresses. The data used to support the findings of this study are available from the corresponding author upon request. A moderate amount of liquid was taken from the tubes and dropped into EMB (HKM, Guangzhou, China) (for K88) and MRS (for P25) media. A�l��H�M�ePe��e�63�X�$���cbڌ !�I�꯴�0jJU&:H'ӻ��U��=g�{Ϲ�;�~�' �|� 0����k4 u���efd���+L��B>Y6T��0��b����Iח���O;�!�ڐ���:�_M+������� u����#�a�����\�~�'h\�?�1Z�&T��P���\��T��OE���#�! From day 7, the number of K88 in PR began to decline, also meaning that P25 had reached the maximum value of 104 CFU/mL per nematode, but K88 in AT continued to grow. Pediococci exert antagonism against pathogens through the production of lactic acid and secretion of bacteriocins, bacteria-killing compounds, known as pediocins. Gene ID: GO function ID. PC1 and PC2 (principal components 1 and 2) in the results of the PCA analysis explained 96% and 2%, respectively, of the distributions of the different groups. 0000004691 00000 n Swenson JM, Facklam RR, Thornsberry C: Antimicrobial susceptibility of vancomycin-resistantLeuconostoc, Pediococcus andLactobacillus species. At the time of positive blood cultures, micafungin and vancomycin were discontinued and the patient was on only piperacillin-tazobactam. These results indicated that P25 is a capable producer of acid. The worms treated with OP50 served as negative controls (CK) while attacked group (AT) worms were treated with OP50 for 20 h; then, as much bacteria as possible were removed from the body surface and gut by submerging in 1 mL M9 medium (0.3% KH2PO4, 0.6% Na2HPO4, 0.5% NaCl, and 0.1% 1 M MgSO4) containing 20 μg/mL levamisole suspension for one minute, six times, and transferred to the K88 treatment.